Transient expression of wild-type and mutant glucocerebrosidases in hybrid vaccinia expression system
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چکیده
منابع مشابه
Expression of wild-type and mutant p53 proteins by recombinant vaccinia viruses
To facilitate the purification of wild type p53 protein, we established a recombinant p53 vaccinia viral expression system. Using this efficient eukaryotic expression vector, we found that the expressed p53 proteins retained their specific structural characteristics. A comparison between wild type and mutant p53 proteins showed the conservation of the typical subcellular localization and the ex...
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SigmaE is a mother cell-specific transcription factor of sporulating Bacillus subtilis that is derived from an inactive precursor protein (pro-sigmaE). To examine the process that prevents sigmaE activity from developing in the forespore, we fused the sigmaE structural gene (sigE) to forespore-specific promoters (PdacF and PspoIIIG), placed these fusions at sites on the B. subtilis chromosome w...
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ژورنال
عنوان ژورنال: European Journal of Human Genetics
سال: 2003
ISSN: 1018-4813,1476-5438
DOI: 10.1038/sj.ejhg.5200974